Obstetrics & Gynaecology
Inogen Vaginal Infections & STIs Fast Real-time PCR Kit
Detects the main causes of vaginal infections and STIs in one fast real-time PCR run, with results in about 45 minutes.
RUO
Reproductive Health
Inogen Sperm DNA Fragmentation Kit
Measures sperm DNA fragmentation to guide male infertility assessment and IVF decisions.
- two sperm variables
- DFI + HDS
- sperm analysed per sample
- ≥5,000
- per test
- 7 to 10 min
- storage
- 2 to 8 °C
01Overview
Overview
Sperm chromatin structure assay principle
Acid denaturation followed by acridine orange staining: intact double-stranded DNA fluoresces green and fragmented single-stranded DNA fluoresces red under 488 nm excitation.
Fast
About 7 to 10 minutes per test with a pre-set flow cytometry programme.
Large sample per test
At least 5,000 sperm are analysed per sample; repeatability CV ≤ 6% and reproducibility CV ≤ 10%.
No liquid nitrogen
A sperm preservation reagent allows storage at 2 to 8 °C for up to 72 hours, −20 °C for 7 days or −80 °C for 30 days.
Targets
- DNA Fragmentation Index (DFI): percentage of sperm with detectable DNA damage
- High DNA Stainability (HDS): percentage of sperm with immature chromatin
Intended use
- Unexplained infertility
- Persistent infertility even after treatment of the female partner
- Couples considering IUI, IVF or ICSI
- Recurrent miscarriage or early pregnancy loss
- Men over 40, men who smoke, or men taking certain prescription medicines that can affect sperm
- Men treated for cancer, exposed to toxic agents or who have had a urinary tract infection
- Men considering varicocele repair
02Specifications
Specifications
- Regulatory status (IFU)
- IVD
- Intended user
- Laboratory technician
- Technology
- Flow cytometry (acridine orange)
- Type of analysis
- Quantitative
- Target
- DNA Fragmentation Index (DFI)
- Validated specimen
- Semen
- Maximum value
- DFI ≤ 98%
- Precision
- Repeatability CV ≤ 6%; reproducibility CV ≤ 10%
- Instrument
- Flow cytometer with 488 nm laser
- Storage
- 2 to 8 °C
03Workflow
Workflow
- 01
Count
Determine sperm density by microscopy or from a semen analysis.
- 02
Denature and stain
Dilute, acid-denature and stain with acridine orange.
- 03
Analyse
Acquire on a flow cytometer with a 488 nm laser and report DFI and HDS.
04Ordering
Ordering
Kit contents
- Reagents A, B and C
- Reagent D (acridine orange)
- Sperm preservation reagent
| Catalogue no. | Kit | Tests | Instrument |
|---|---|---|---|
PI0100050 | Inogen Sperm DNA Fragmentation Kit | 50 | Flow cytometer with 488 nm laser |
PI0100100 | Inogen Sperm DNA Fragmentation Kit | 100 | Flow cytometer with 488 nm laser |
05Documents
Documents
Related products
Antenatal screening
Inogen I-GBS Real-time PCR Kit
Antenatal screening for Group B Streptococcus, helping protect newborns from early infection.
IVD
Questions about a kit?
Our team answers laboratory and procurement enquiries in English and Vietnamese.